2012 Workshop

LBNL Integrated Bioimaging Initiative Workshop

Thursday, February 2, 2012
8:30 a.m. - 6:30 p.m. (incl. reception)
717 Potter Street, room 141
Berkeley, CA 97710


The goals of this workshop were to:
- Bring together people with expertise in current equipment/technologies at the Lab/UCB that span the imaging spectrum.
- Identify current equipment/technologies inventory and needs.
- Identify opportunities and challenges in integrated bioimaging.
- Use the outcome to develop a "white paper" to obtain large-scale funding.

Registration Required - Now Closed


8:30       Welcome and introduction - by Gary Karpen
Bioimaging capabilities - intros by experts across the Lab
Diffractive imaging, SAXS - imaging/measuring molecular structure and dynamics - John Tainer
CryoEM, TEM, (FIB/)SEM, EM tomography - imaging molecules and ultrastructure - Eva Nogales
FTIR, Raman, and other imaging spectroscopies - imaging chemical composition - Jim Schuck
TOF-SIMS, NIMS, MALDI-MSI, and other mass-spec imaging - imaging the omics - Trent Northen
Fluorescence, confocal, super-resolution, etc. microscopies - imaging (sub)cellular systems and tissues - Jan Liphardt
Medical imaging technologies and radiochemistry, MRI, PET, SPECT - imaging whole organisms - Bill Jagust
10:15     Break
Bioimaging capabilities - intros by experts across the Lab ~ continued

Sub-surface imaging and geo-biology imaging - imaging biology in the environment - Peter Nico
AFM, NSOM, and other scanning probe microscopies - imaging and manipulating biologicy at the surface - Dan Fletcher
Inorganic labels, qdots, plasmon-rulers - inorganic multi-modality probes for imaging - Bruce Cohen

Visualization and visualization-driven analysis - helping scientists understand data derived from imaging - Wes Bethel

Image analysis techniques - turning images into data - James Sethian

Organic and genetically expressed labels and biosensors - organic multi-modality probes for imaging - Gerard Marriott

11:45     Announcement break-out sessions and assignments
Noon      Working lunch
1:00       Break-out sessions (working groups*)
2:30       Break
3:00       Working groups report on their findings
5:00       Steps going forward - by Gary Karpen
5:30       Reception

* Questions addressed by the working groups:
A) How can we measure and map communication between individual cells in microbial communities and/or eukaryotic tissues that are required to perform their communal functions?
B) How can we measure the effects of environmental impacts (radiation, stresses, chemicals, epigenomic factors) on genome, cellular, and organismal functions?
C) How can we comprehensively extract tissue-level, cellular and sub-cellular information both from primary patient material and in-vivo for diagnostics and evaluation of therapeutic effectiveness?
D) What capabilities can we bring together to comprehensively image the structure, dynamics, and function of a living cell at a molecular resolution?
E) How can we simultaneous and dynamically image/measure the chemical and physical environmental and microbial metabolism at the 'microbial scale' in order to understand physical-chemical-metabolic feed-backs?

The introduction presentation by Gary Karpen and several of the bioimaging capabilities presentations (some edited for sharing) are attached below.

Introduction Presentations:
Introduction slides of workshop participants are attached below for your perusal.


For more information contact bioimaging@lbl.gov.

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